The mitochondrial sequence variations were subjected to phylogenetic haplogroup assessment and pathogenicity of the mutations were predicted using pMUT and PolyPhen2. The entire mitochondrial genome was sequenced using the AffymetrixGeneChip Human Mitochondrial Resequencing Array 2.0. Relative oxidative stress level, mitochondria activity, and mitochondrial mass were determined by live cell imaging with confocal laser scanning microscope using CM-H 2DCFDA, MitoTracker Green, and MitoTracker Orange stains. Three different grades of human glioma cell lines and a normal human astrocyte cell line were cultured in-vitro and tested for oxidative stress biomarkers.
This study aimed to identify mitochondrial DNA (mtDNA) sequence variations that could possibly affect the mitochondrial functions and also the oxidative stress status. However, the role of mitochondria in different grade gliomasis still unknown. The role of mitochondria in tumorigenesis has regained much attention as it could dysregulate cellular energetics, oxidative stress and apoptosis. 5Department of Community Health, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.4Neurosurgery Department, Hospital Kuala Lumpur, Kuala Lumpur, Malaysia.3The University of Nottingham Malaysia Campus, Semenyih, Malaysia.2Division of Neurosurgery, Department of Surgery, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.1UKM Medical Molecular Biology Institute, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.Mohd Haspani 4, Charng Jeng Toh 2, Azmi Mohd Tamil 5, Roslan Harun 1, Wan Z. Bee Hong Soon 1,2, Nor Azian Abdul Murad 1 *, Sue-Mian Then 1,3, Azizi Abu Bakar 2, Farizal Fadzil 2, Jegan Thanabalan 2, Mohd S.
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